The immunoturbidimetry method is an analytical technique combining liquid-phase precipitation reactions with optical instruments and automated analysis. However, due to issues relating to the size and quantity of antigen-antibody complexes, the immunoturbidimetry has low sensitivity and a narrow detection range. This has led to the development of the latex immunoturbidimetric assay (LIA). This technique uses latex microparticles that are coated with specific antibodies or antigens.

When these particles encounter the target analyte in a sample, they form specific aggregates through antigen-antibody interactions. The aggregation of these particles increases the scattering intensity or absorbance of the latex suspension. This increase is quantitatively correlated with the concentration of the target analyte in the sample. Turbidity measurements are performed using a biochemical analyser, and the entire analytical process takes only a few minutes. Compared to traditional immunoturbidimetric methods, this method offers significantly higher sensitivity, with detection limits as low as ng/mL or pg/mL.

CD Bioparticles has extensive experience in assay solutions and now offers custom services to help researchers and diagnostic product developers develop latex immunoturbidimetric assays. The company's service portfolio ranges from custom conjugation to the optimization of complete immunoturbidimetric assays. The company provides custom-made, carboxyl-functionalized polystyrene microspheres that are conjugated with antigens or antibodies and come in various sizes. CD Bioparticles also offers complete kits for the quantitative detection of a wide range of biomarkers. These products feature high sensitivity, short assay times and compatibility with automated biochemical analyzers.

CD Bioparticles offers customers carboxyl-modified polystyrene microparticles in a range of sizes (from 0.07 to 0.4 μm) that are suitable for developing latex immunoturbidimetric assay reagents. CD Bioparticles' standard products feature a moderate surface carboxyl density; products with higher or lower densities are also available upon request. Additionally, CD Bioparticles can customize the conjugation of antibodies and antigens to meet specific application requirements. Through optimization, the team can ensure high conjugation efficiency while maintaining the activity of both antibodies and antigens.

CD Bioparticles also offers reagent formulation development and optimization services to help customers improve reagent stability, optimize reaction conditions and enhance detection sensitivity. The company offers performance validation services to verify key performance indicators, including linearity, sensitivity, specificity and precision, ensuring stability across different batches and under various operating conditions.

For more information about CD Bioparticles' Custom Latex Immunoturbidimetric Assay development services or to submit a project inquiry, please visit https://www.cd-bioparticles.com/services/latex-immunoturbidimetric-assay-development.html.

About CD Bioparticles

CD Bioparticles is a leading manufacturer and supplier of various nanoparticles, microparticles, and coatings for R&D as well as commercialization across different application areas, including in vitro diagnostics, biochemistry, cellular analysis, cell separation, and immunoassay. The company also offers various custom services, including chemical surface-functionalization, fluorescent modification, antibody immobilization, as well as nucleic acid and oligo conjugation to meet client specifications.